Epigenomic Reprogramming toward Mesenchymal-Epithelial Transition in Ovarian-Cancer-Associated Mesenchymal Stem Cells Drives Metastasis
Copyright policy )Epigenomic Reprogramming toward Mesenchymal-Epithelial Transition in Ovarian-Cancer-Associated Mesenchymal Stem Cells Drives Metastasis
A role for cancer cell epithelial-to-mesenchymal transition (EMT) in cancer is well established. Here, we show that, in addition to cancer cell EMT, ovarian cancer cell metastasis relies on an epigenomic mesenchymal-to-epithelial transition (MET) in host mesenchymal stem cells (MSCs). These reprogrammed MSCs, termed carcinoma-associated MSCs (CA-MSCs), acquire pro-tumorigenic functions and directly bind cancer cells to serve as a metastatic driver/chaperone. Cancer cells induce this epigenomic MET characterized by enhancer-enriched DNA hypermethylation, altered chromatin accessibility, and differential histone modifications. This phenomenon appears clinically relevant, as CA-MSC MET is highly correlated with patient survival. Mechanistically, mirroring MET observed in development, MET in CA-MSCs is mediated by WT1 and EZH2. Importantly, EZH2 inhibitors, which are clinically available, significantly inhibited CA-MSC-mediated metastasis in mouse models of ovarian cancer.
- University of Pittsburgh United States
- Johns Hopkins University United States
- Johns Hopkins Medicine United States
- Johns Hopkins University Sch of Medicine United States
- University of Pittsburgh Cancer Institute United States
Epigenomics, Ovarian Neoplasms, Epithelial-Mesenchymal Transition, Primary Cell Culture, Gene Expression, Cell Differentiation, Mesenchymal Stem Cells, Carcinoma, Ovarian Epithelial, Article, Gene Expression Regulation, Neoplastic, Epigenome, Mice, Cell Movement, Mice, Inbred NOD, Cell Line, Tumor, Animals, Humans, Enhancer of Zeste Homolog 2 Protein, Female, Neoplasm Metastasis, Cell Proliferation
Epigenomics, Ovarian Neoplasms, Epithelial-Mesenchymal Transition, Primary Cell Culture, Gene Expression, Cell Differentiation, Mesenchymal Stem Cells, Carcinoma, Ovarian Epithelial, Article, Gene Expression Regulation, Neoplastic, Epigenome, Mice, Cell Movement, Mice, Inbred NOD, Cell Line, Tumor, Animals, Humans, Enhancer of Zeste Homolog 2 Protein, Female, Neoplasm Metastasis, Cell Proliferation
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