Analysis of the transcriptional regulation of the FABP2 promoter haplotypes by PPARγ/RXRα and Oct-1
Copyright policy )Analysis of the transcriptional regulation of the FABP2 promoter haplotypes by PPARγ/RXRα and Oct-1
Variants of the human intestinal fatty acid binding protein 2 gene (FABP2) are associated with traits of the metabolic syndrome. Relevant FABP2 promoter polymorphisms c.-80_-79insT, c.-136_-132delAGTAG, c.-168_-166delAAGinsT, c.-260G>A, c.-471G>A, and c.-778G>T result in two haplotypes A and B. Activation of haplotypes by rosiglitazone stimulated PPARgamma/RXRalpha leads to 2-fold higher activity of haplotype B than A. As shown by chimeric FABP2 promoter constructs, the higher responsiveness of FABP2 haplotype B is mainly but not solely determined by polymorphism c.-471G>A. As shown by EMSA and promoter-reporter assays, Oct-1 interacts with the -471 region of FABP2 promoters, induces the activities of both FABP2 promoter haplotypes and abolishes the different activities of haplotypes induced by rosiglitazone activated PPARgamma/RXRalpha. In conclusion, our findings suggest a functional role of PPARgamma/RXRalpha and Oct-1 in the regulation of the FABP2 gene.
- Kiel University Germany
Binding Sites, Polymorphism, Genetic, Retinoid X Receptor alpha, Transcription, Genetic, Fatty Acid-Binding Proteins, Transfection, PPAR gamma, Rosiglitazone, Gene Expression Regulation, Haplotypes, Humans, Protein Isoforms, Thiazolidinediones, Caco-2 Cells, Promoter Regions, Genetic, HeLa Cells, Octamer Transcription Factor-1, Protein Binding
Binding Sites, Polymorphism, Genetic, Retinoid X Receptor alpha, Transcription, Genetic, Fatty Acid-Binding Proteins, Transfection, PPAR gamma, Rosiglitazone, Gene Expression Regulation, Haplotypes, Humans, Protein Isoforms, Thiazolidinediones, Caco-2 Cells, Promoter Regions, Genetic, HeLa Cells, Octamer Transcription Factor-1, Protein Binding
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