Transient expression of Arabidopsis thaliana ascorbate peroxidase 3 in Nicotiana benthamiana plants infected with recombinant potato virus X
Copyright policy )Transient expression of Arabidopsis thaliana ascorbate peroxidase 3 in Nicotiana benthamiana plants infected with recombinant potato virus X
We have explored the transient over-expression of Arabidopsis thaliana ascorbate peroxidase 3 (APX3) in Nicotiana benthamiana using a viral vector based on the potato virus X (PVX). Plants infected with a PVX:APX3 hybrid had a similar progression of viral particles compared to control plants infected with a PVX:GFP hybrid, indicating that infection was not affected by the over-expression of heterologous APX3. Our results also showed that in PVX:APX3-infected plants, the hybrid virus directed a high level of APX3 expression and the recombinant protein was functional, as inferred from the higher APX activity compared to mock and PVX:GFP hybrid-infected plants. The PVX recombinant expression system used is a simple and quick method for transient expression of heterologous APXs, which are expected to suffer specific processing in plant cells.
- John Innes Centre United Kingdom
- Sainsbury Laboratory United Kingdom
- University of Castile-La Mancha Spain
- Biotechnology and Biological Sciences Research Council United Kingdom
Nicotiana, Arabidopsis Proteins, Genetic Vectors, Immunoblotting, Arabidopsis, Membrane Proteins, Blotting, Northern, Plants, Genetically Modified, Gene Expression Regulation, Enzymologic, Potexvirus, Chloroplast Proteins, Ascorbate Peroxidases, Peroxidases, Gene Expression Regulation, Plant, RNA, Plant
Nicotiana, Arabidopsis Proteins, Genetic Vectors, Immunoblotting, Arabidopsis, Membrane Proteins, Blotting, Northern, Plants, Genetically Modified, Gene Expression Regulation, Enzymologic, Potexvirus, Chloroplast Proteins, Ascorbate Peroxidases, Peroxidases, Gene Expression Regulation, Plant, RNA, Plant
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