Intracisternal A-type particle elements as genetic markers: detection by repeat element viral element amplified locus-PCR
Copyright policy )Intracisternal A-type particle elements as genetic markers: detection by repeat element viral element amplified locus-PCR
We describe a novel, PCR-based technique termed REVEAL-PCR for examining the inheritance of intracisternal A-type particles (IAP). Amplifications use an unlabeled primer to SINE repeats and a radiolabeled primer to the IAP long terminal repeat; labeled products, which can be resolved on sequencing gels, are formed when IAPs lie in proximity to SINEs. With this technique we have identified a total of 124 polymorphisms in the BXH and CXS recombinant inbred strains. We suggest that this method will be equally applicable for examining other gene families present at around a thousand copies per genome.
- Tanzania Commission for Science and Technology Tanzania (United Republic of)
- National Institute for Medical Research Tanzania (United Republic of)
- National Institute for Medical Research United Kingdom
Genetic Markers, Mice, Inbred BALB C, Mice, Inbred C3H, Base Sequence, Molecular Sequence Data, Polymerase Chain Reaction, Leukemia Virus, Murine, Mice, Inbred C57BL, Mice, Genes, Intracisternal A-Particle, Proviruses, Animals, Feasibility Studies, Repetitive Sequences, Nucleic Acid
Genetic Markers, Mice, Inbred BALB C, Mice, Inbred C3H, Base Sequence, Molecular Sequence Data, Polymerase Chain Reaction, Leukemia Virus, Murine, Mice, Inbred C57BL, Mice, Genes, Intracisternal A-Particle, Proviruses, Animals, Feasibility Studies, Repetitive Sequences, Nucleic Acid
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