Induction of Herpes Simplex Virus gB-Specific Cytotoxic T Lymphocytes in TAP1-Deficient Mice by Genetic Immunization but Not HSV Infection
Copyright policy )Induction of Herpes Simplex Virus gB-Specific Cytotoxic T Lymphocytes in TAP1-Deficient Mice by Genetic Immunization but Not HSV Infection
Loading of most endogenous peptides on major histocompatibility complex class I molecules is conditional on their transport into the endoplasmic reticulum (ER) by the peptide transporter TAP. We describe an HSV-2/1 cross-reactive cytotoxic T-cell (CTL) epitope that is processed in a TAP1-independent manner in vivo following immunization of TAP1-/- mice with naked DNA or a recombinant vaccinia virus. These data indicated that TAP1-independent processing of endogenous proteins is sufficient to prime CTLs in vivo. TAP1-independent processing of this epitope was not due to ER targeting by signal sequences and exogenous loading of MHC-I molecules and was not influenced by the amino acids flanking this epitope. In contrast, TAP1-/- mice infected with HSV-2 or HSV-2 mutants did not mount a CTL response against this epitope.
- Chiron Corporation United States
Mice, Knockout, Antigen Presentation, Extracellular Matrix Proteins, Epitopes, T-Lymphocyte, Herpes Simplex, Nerve Tissue Proteins, Vaccinia virus, Viral Vaccines, Cross Reactions, Cell Line, Mice, Inbred C57BL, Mice, Viral Envelope Proteins, Virology, Vaccines, DNA, Animals, Simplexvirus, Female, Immunization, T-Lymphocytes, Cytotoxic
Mice, Knockout, Antigen Presentation, Extracellular Matrix Proteins, Epitopes, T-Lymphocyte, Herpes Simplex, Nerve Tissue Proteins, Vaccinia virus, Viral Vaccines, Cross Reactions, Cell Line, Mice, Inbred C57BL, Mice, Viral Envelope Proteins, Virology, Vaccines, DNA, Animals, Simplexvirus, Female, Immunization, T-Lymphocytes, Cytotoxic
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