Genomic Organization of the CC Chemokine MIP-3α/CCL20/LARC/EXODUS/SCYA20, Showing Gene Structure, Splice Variants, and Chromosome Localization
Copyright policy )Genomic Organization of the CC Chemokine MIP-3α/CCL20/LARC/EXODUS/SCYA20, Showing Gene Structure, Splice Variants, and Chromosome Localization
We describe the genomic organization of a recently identified CC chemokine, MIP3alpha/CCL20 (HGMW-approved symbol SCYA20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. Two distinct cDNAs were identified, encoding two forms of MIP-3alpha/CCL20, Ala MIP-3alpha/CCL20 and Ser MIP-3alpha/CCL20, that differ by one amino acid at the predicted signal peptide cleavage site. Examination of the sequence around the boundary of intron 1 and exon 2 showed that use of alternative splice acceptor sites could give rise to Ala MIP-3alpha/CCL20 or Ser MIP-3alpha/CCL20. Both forms of MIP-3alpha/CCL20 were chemically synthesized and tested for biological activity. Both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha/CCL20. T lymphocytes exposed only to IL-2 responded inconsistently, while no response was detected in naive T lymphocytes, monocytes, or neutrophils. The biological activity of Ser MIP-3alpha/CCL20 and Ala MIP-3alpha/CCL20 and the tissue-specific preference of different splice acceptor sites are not yet known.
- University of Queensland Australia
- University of Queensland Australia
- University of Adelaide Australia
- Princess Alexandra Hospital Australia
- Pfizer (United States) United States
Receptors, CCR6, Insitu Hybridization, T-Lymphocytes, Molecular Sequence Data, 610, Chromosome, 730102 Immune system and allergy, C1, Cc Chemokine, Humans, Cloning, Molecular, Cytokine, In Situ Hybridization, Fluorescence, Genetics & Heredity, Gene Structure, Inflammatory Protein 3-alpha, Chemokine CCL20, Base Sequence, T-cells, Chromosome Mapping, Dendritic Cells, DNA, Exons, Macrophage Inflammatory Proteins, Introns, Boyden Chamber, Alternative Splicing, Biotechnology & Applied Microbiology, Beta-chemokine, Chemokines, CC, Chromosomes, Human, Pair 2, 320200 Immunology, Calcium, Receptors, Chemokine, Polycarbonate Nuclepore Membranes, Receptor, Cloning
Receptors, CCR6, Insitu Hybridization, T-Lymphocytes, Molecular Sequence Data, 610, Chromosome, 730102 Immune system and allergy, C1, Cc Chemokine, Humans, Cloning, Molecular, Cytokine, In Situ Hybridization, Fluorescence, Genetics & Heredity, Gene Structure, Inflammatory Protein 3-alpha, Chemokine CCL20, Base Sequence, T-cells, Chromosome Mapping, Dendritic Cells, DNA, Exons, Macrophage Inflammatory Proteins, Introns, Boyden Chamber, Alternative Splicing, Biotechnology & Applied Microbiology, Beta-chemokine, Chemokines, CC, Chromosomes, Human, Pair 2, 320200 Immunology, Calcium, Receptors, Chemokine, Polycarbonate Nuclepore Membranes, Receptor, Cloning
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