The Human and Mouse Period1 Genes: Five Well-Conserved E-Boxes Additively Contribute to the Enhancement of mPer1 Transcription
Copyright policy )The Human and Mouse Period1 Genes: Five Well-Conserved E-Boxes Additively Contribute to the Enhancement of mPer1 Transcription
The clock gene, Period1, from human and mouse was sequenced and characterized. Both human PERIOD1 (human PER1) and mouse Period1 (mouse Per1) consisted of 23 exons spanning approximately 16 kb, and their structures showed strong similarity to each other. For example, six highly conserved regions were identified in the 5' upstream sequences. These conserved segments exhibited 77-88% identity and possessed several potential regulatory elements including five E-boxes (the binding site of the CLOCK-BMAL1 complex) and four cyclic AMP response elements. Transient transfection assays using a mPer1-luciferase fusion gene revealed that each of the conserved E-boxes additively functions as an enhancer for the transactivation of mPer1 by mCLOCK and mBMAL1.
- RIKEN Japan
- University of Tokyo Japan
Binding Sites, Base Sequence, Molecular Sequence Data, ARNTL Transcription Factors, CLOCK Proteins, Nuclear Proteins, Cell Cycle Proteins, Period Circadian Proteins, Sequence Analysis, DNA, Response Elements, Circadian Rhythm, Mice, Basic Helix-Loop-Helix Transcription Factors, Cyclic AMP, Trans-Activators, Animals, Humans, Amino Acid Sequence, Promoter Regions, Genetic, Conserved Sequence
Binding Sites, Base Sequence, Molecular Sequence Data, ARNTL Transcription Factors, CLOCK Proteins, Nuclear Proteins, Cell Cycle Proteins, Period Circadian Proteins, Sequence Analysis, DNA, Response Elements, Circadian Rhythm, Mice, Basic Helix-Loop-Helix Transcription Factors, Cyclic AMP, Trans-Activators, Animals, Humans, Amino Acid Sequence, Promoter Regions, Genetic, Conserved Sequence
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).131 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!