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Transcriptional Regulation of Muscle-Specific Genes during Myoblast Differentiation

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1998 English Publisher:Elsevier BVJournal:Biochemical and Biophysical Research Communications, volume 246, pages 287-292 (issn: 0006-291X,

Authors: Teruhiko Shimokawa; Seiichi Hashimoto; Osamu Ezaki; Miyuki Kato;

doi: 10.1006/bbrc.1998.8600

pmid: 9600108

Transcriptional Regulation of Muscle-Specific Genes during Myoblast Differentiation

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Abstract

A rapid, highly sensitive method to determine the mRNA level of muscle-specific markers using TaqMan PCR analysis was developed and used to study sequential gene regulation of myoblasts during induced differentiation of C2C12 cells. mRNA levels of muscle regulatory factor (MRF) myogenin, alpha-actin, thermoregulatory uncoupling protein UCP2 and glucose transporter isotype glut4 increased rapidly during early stage differentiation. In contrast, myf5, beta-actin, UCP1 and glut1 mRNA levels gradually decreased during 8 days. However, the mRNA level of other genes such as MyoD, glyceraldehyde-3-phosphate dehydrogenase and hexokinase II changed only slightly in comparison. Muscle-specific uncoupling protein UCP3 mRNA was detected during differentiation and increased rapidly within 8 days. These data clearly show the sequential and the differential regulation of muscle-specific genes in C2C12 cells during multinucleate myotube formation.

Related Organizations

Laboratory for Personalized Molecular Medicine
United States

Keywords

DNA, Complementary, Base Sequence, Muscles, Molecular Sequence Data, Gene Expression Regulation, Developmental, Muscle Proteins, Cell Differentiation, Polymerase Chain Reaction, Sensitivity and Specificity, Cell Line, Kinetics, Mice, Myogenic Regulatory Factors, Animals, RNA, Messenger, DNA Primers

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	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	94
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	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%