A Single B1 Subunit Mapped to Mouse Chromosome 7 May Be a Common Component of Na Channel Isoforms from Brain, Skeletal Muscle and Heart
Copyright policy )A Single B1 Subunit Mapped to Mouse Chromosome 7 May Be a Common Component of Na Channel Isoforms from Brain, Skeletal Muscle and Heart
A beta 1 subunit associated with one or more isoforms of brain voltage-sensitive Na channels has previously been cloned, sequenced and expressed. Northern and Western blot analyses have suggested that homologues to this protein are expressed in skeletal muscle and heart. Here, reverse transcriptase-polymerase chain reaction (RT-PCR)/cloning reveals that transcripts encoding identical beta 1 subunit ORFs are expressed in adult rat brain, skeletal muscle and heart. Heterologous co-expression of beta 1 with brain (RIIA) and skeletal muscle (mu 1) alpha subunits caused a stabilization of normal, rapidly inactivating (mode 1) gating relative to anomalous, non-inactivating (mode 2) states and a negative shift in steady state inactivation. Chromosome mapping of the beta 1 subunit showed a single locus (Scn1b) in mouse chromosome 7 1.8 cM (+/- 1.2 cM) distal to D19F11S1h and 0.9 cM (+/- 0.9 cM) proximal to Pkca. This locus is in the region of the mouse mutant "quivering," characterized by a variety of neurological disorders and muscle paralysis. A mutation in a single beta 1 subunit forming functional complexes with multiple Na channel isoforms could underlie these deficits.
- Johns Hopkins University United States
- Johns Hopkins Medicine United States
- Duke University United States
Base Sequence, Macromolecular Substances, Muscles, Myocardium, Molecular Sequence Data, Brain, Chromosome Mapping, Membrane Proteins, Polymerase Chain Reaction, Membrane Potentials, Muridae, Kinetics, Mice, Mice, Neurologic Mutants, Open Reading Frames, Oligodeoxyribonucleotides, Organ Specificity, Oocytes, Animals, Crosses, Genetic
Base Sequence, Macromolecular Substances, Muscles, Myocardium, Molecular Sequence Data, Brain, Chromosome Mapping, Membrane Proteins, Polymerase Chain Reaction, Membrane Potentials, Muridae, Kinetics, Mice, Mice, Neurologic Mutants, Open Reading Frames, Oligodeoxyribonucleotides, Organ Specificity, Oocytes, Animals, Crosses, Genetic
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