The spectrum of Trp− mutants isolated as 5‐fluoroanthranilate‐resistant clones in Saccharomyces bayanus, S. mikatae and S. paradoxus
Copyright policy )The spectrum of Trp− mutants isolated as 5‐fluoroanthranilate‐resistant clones in Saccharomyces bayanus, S. mikatae and S. paradoxus
Abstract5‐Fluoroanthranilic acid (FAA)‐resistant mutants were selected in homothallic diploids of three Saccharomyces species, taking care to isolate mutants of independent origin. Mutations were assigned to complementation groups by interspecific complementation with S. cerevisiae tester strains. In all three species, trp3, trp4 and trp5 mutants were recovered. trp1 mutants were also recovered if the selection was imposed on a haploid strain. Thus, FAA selection may be more generally applicable than was previously described. Copyright © 2007 John Wiley & Sons, Ltd.
- Carnegie Mellon University United States
Fungal Proteins, Saccharomyces, Genetic Complementation Test, Mutation, Indole-3-Glycerol-Phosphate Synthase, Tryptophan, ortho-Aminobenzoates, Aldose-Ketose Isomerases, Anthranilate Synthase
Fungal Proteins, Saccharomyces, Genetic Complementation Test, Mutation, Indole-3-Glycerol-Phosphate Synthase, Tryptophan, ortho-Aminobenzoates, Aldose-Ketose Isomerases, Anthranilate Synthase
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