Generation of Nkx2.2:lacZ mice using recombination‐mediated cassette exchange technology
Copyright policy )Generation of Nkx2.2:lacZ mice using recombination‐mediated cassette exchange technology
AbstractNkx2.2 encodes a homeodomain transcription factor required for the correct specification and/or differentiation of cells in the pancreas, intestine, and central nervous system (CNS). To follow the fate of cells deleted for Nkx2.2 within these tissues, we generated Nkx2.2:lacZ knockin mice using a recombination‐mediated cassette exchange (RMCE) approach. Expression analysis of lacZ and/or β‐galactosidase in Nkx2.2lacZ/+ heterozygote embryos and adults demonstrates that lacZ faithfully recapitulates endogenous Nkx2.2 expression. Furthermore, the Nkx2.2lacZ/lacZ homozygous embryos display phenotypes indistinguishable from the previously characterized Nkx2.2−/− strain. LacZ expression analyses in the Nkx2.2lacZ/lacZ homozygous embryos indicate that Nkx2.2‐expressing progenitor cells within the pancreas are generated in their normal numbers and are not mislocalized within the pancreatic ductal epithelium or developing islets. In the CNS of Nkx2.2lacZ/lacZ embryos, LacZ‐expressing cells within the ventral P3 progenitor domain display different migration properties depending on the developmental stage and their respective differentiation potential. genesis 50:612–624, 2012. © 2012 Wiley Periodicals, Inc.
- King’s University United States
- Columbia University United States
- Vanderbilt University United States
Central Nervous System, Homeodomain Proteins, Genotype, Gene Expression Regulation, Developmental, Mice, Transgenic, Zebrafish Proteins, beta-Galactosidase, Mice, Homeobox Protein Nkx-2.2, Phenotype, Neural Stem Cells, Gene Order, Gene Targeting, Animals, Homologous Recombination, Pancreas, Transcription Factors
Central Nervous System, Homeodomain Proteins, Genotype, Gene Expression Regulation, Developmental, Mice, Transgenic, Zebrafish Proteins, beta-Galactosidase, Mice, Homeobox Protein Nkx-2.2, Phenotype, Neural Stem Cells, Gene Order, Gene Targeting, Animals, Homologous Recombination, Pancreas, Transcription Factors
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