Cell biology is in the midst of a remarkable “resolution revolution” in which the invention of new optical microscope techniques is allowing visualisation of the dynamics of intra-cellular structures at unprecedented spatial and temporal resolution. We are requesting a Lattice Light Sheet Microscope (LLSM) as developed by Eric Betzig (Janelia) - recipient of the 2014 Nobel Prize for his earlier work on super-resolution microscopy - and a dedicated microscope technician. The LLSM enables simultaneous illumination of the entire field of view with an ultrathin sheet of excitation light permitting imaging at hundreds of planes per second with high axial resolution (~230-nm xy and ~370-nm z), negligible out-of-focus background and a dramatic reduction in photobleaching/toxicity/damage. The LLSM thus opens up a completely new horizon, enabling long-term interrogation of the mechanisms of cell biological machines, inside live cells. Our aim is to make this technology available to Warwick’s expanding cell and developmental biological community - nucleated by four Wellcome Investigators (McAinsh, Straube, Balasubramanian & Cross) based in the Centre for Mechanochemical Cell Biology. We will also establish a visitor programme - inspired by the advanced imaging centre at Janelia - to make this technology accessible to the wider community.