Our research grouping has inter-disciplinary expertise in determining the mechanisms by which signal transduction modulates cell and organ behaviour under normal and disease conditions. The functional roles of living biological systems are controlled by the integration of many different molecular pathways. These can be incredibly complicated to understand whether one is considering events at the single cell, interactions between cells in complex multicellular systems or even between organs. T hese processes are finitely regulated in both time and space. Our objectives are to utilise state-of-the-art bioimaging equipment to advance our understanding of spatiotemporal signalling dynamics at different system levels in vitro and in vivo. In particular: (i) the use of fast spinning disk laser scanning fluorescent microscopy with photoactivation (flash photolysis, FRAP and FRET) and electrophysiology to study the molecular cell physiology with the goal of increasing our understanding ho w individual cells process biological information. (ii) whole body 3-D bioluminescent and biofluorescent imaging to track cellular movement and activation between different organs with the aim of developing new understandings of disease progression. In combination the equipment will enable the investigation of signalling events from cells to tissue to whole body, from in vitro to in vivo.