The genomics revolution has opened up the protein world for discovery research in medicine, biosciences and biotechnology. Interpreting and exploiting this information necessitates an understanding of how proteins function as part of complex and dynamic macro-molecular assemblies in cells. Single domain antibodies or nanobodies are powerful tools for tagging and tracking proteins and their interactions. They can lock proteins into specific shapes that makes it easier to study their structure. By making nanobody technology more widely available to the UK Biosciences research community, the Nanobody Discovery Hub project will help accelerate research into how proteins work and how this knowledge can be usefully applied in structural, cell and synthetic biology.