My proposal presents a strategy to set up the first minimal system of mRNA export through a biomimetic nuclear pore complex (NPC), which will allow to resolve crucial questions on nuclear export. Separation of genetic material in the cell nucleus allows mRNA to be modified before export to the cytoplasm for translation. When these modifications have been completed, NPCs (that maintain a selective barrier for the transport of macromolecules between the nuclear and cytoplasmic compartments) provide a highly selective channel through the nuclear envelope. Whereas proteins are imported into the nucleus after translation in the cytoplasm, mRNAs are exported after transcription and processing in the nucleus is finished. Unlike protein import, mRNA export proceeds by addition and removal of mRNA-binding proteins until an export-competent messenger ribonucleoprotein particle (mRNP) is formed by attaching the heterodimeric Mex67/Mtr2 transport factor. Crucial questions in this fields are: what is the mechanism by which Mex67/Mtr2 mediates translocation? What are the minimal requirements for transport? What is the Mex67/Mtr2/mRNA stoichiometry within mRNPs? Which conformations do mRNP complexes assume during transport? The molecular complexity of NPCs has defeated classical cellular and molecular approaches addressing such questions. I propose to tackle them using the powerful, innovative setup offered by the biomimetic NPCs, minimal NPCs obtained by coating a solid-state nanopore with NPC transport channel components. This functional artificial pore can deliver powerful information about translocations at a single-molecule level and has already been used successfully to study protein import. I propose, for the first time, to tailor biomimetic nanopores to study mRNA export dynamics to help disentangle mRNPs translocation from their production, maturation, and disassembling steps, allowing key transport questions to be addressed. This minimal pore system will enable the nature of the minimal mRNP export mechanism to be unravelled, and presents unique opportunities for fine-tuned control.