Biological engineers are developing increasingly sophisticated technical measures intended to mitigate environmental, safety and security effects of synthetic biology. Research on methods of intrinsic biocontainment have progressed from simple kill switches to multiple nutrient dependency strategies to reduce fitness and engineered genetic codes to limit horizontal gene transfer. Research to date has focused on lab strains of E.coli rather than commercial chassis as model organisms. Furthermore, containment methods have not been the objects of independent technical testing or of systematic multistakeholder informed risk assessments. The proposed research and utilization plan is designed to fill these gaps in existing work and will pave the way for a systematic risk assessment and, where applicable, risk management of microbes of industrial interest.