Parkinson’s disease (PD) is one of the most common neurodegenerative disorders, caused by aggregation of the protein alpha-synuclein (αSyn). Understanding how and when αSyn aggregates form remains a major challenge in comprehending PD pathology, as there is currently no method to visualize the early stages of aggregation. We propose to develop an innovative method to study αSyn aggregation under near native conditions in human neurons. Using a novel protein labelling technique we aim to monitor real-time aggregation inside living cells, which could pave the way for new therapies targeting the early phases of PD.