The BIOBESTicide project will validate and demonstrate the production of an effective and cost-efficient biopesticide. The demonstration will be based on an innovative bio-based value chain starting from the valorisation of sustainable biomasses, i.e. beet pulp and sugar molasses and will exploit the properties of the oomycete Pythium oligandrum strain I-5180 to increase natural plant defenses, to produce an highly effective and eco-friendly biopesticide solution for vine plants protection. GREENCELL, the project coordinator, has developed, at laboratory level (TRL4), an effective method to biocontrol one of the major causes of worldwide vineyards destruction, the Grapevine Trunk Diseases (GTDs). The protection system is based on the oomycete Pythium oligandrum strain I-5180 that, at applied at optimal time and concentration, colonises the root of vines and stimulates the natural plant defences against GTDs, providing a protection that ranges between 40% and 60%. BIOBESTicide project will respond to the increasing demands for innovative solutions for crop protection agents, transferring the technology to a DEMO Plant able to produce more than 10 T of a high-quality oomycete-based biopesticide product per year (TRL7). The BIOBESTicide project will validate the efficiency of the formulated product on vineyards of different geographical areas. To assure the safety of products under both health and environmental points of view, a full and complete approval dossier for Pythium oligandrum strain I-5180 will be submitted in all the European countries. A Life Cycle Sustainability Assessment (LCSA) will be conducted to assess the environmental, economic and social impacts of the developed products. The adoption of the effective and cost-efficient biopesticide will have significant impacts with a potential ROI of 30 % in just 5 years and a total EBITDA of more than € 6,400,000.