New strategies of the European Union related with transition to bio-based and circular economy involve several sustainability requirements, among others, at the environmental level. In this context, the project Bio-Uptake targeted the development of bio-based polymers with innovation towards circular solutions, promoting the re-use of the products to be developed (bath ceiling, foot orthosis, garbage lid). In Bio-Uptake-Hop-On, UAveiro brings to Bio-Uptake, a new pillar to fully achieve the environmental sustainability of the developed products, by certifying their low environmental and human impacts. Thus, Bio-Uptake-Hop-On main goal is to characterize the safety of developed bio-based materials and products to Environmental and Human (E&H) Health, across the different production and innovation steps. For this, three fit-for-purpose approaches will apply: i) assess the E&H hazardousness of the materials and products, to support applying the safe-by-design concept and select the least hazardous materials and intermediate product formats to proceed to the final production stage; ii) identify the best architecture design of foot orthosis and bath ceiling formats that promotes less growth of human pathogens, and (iii) characterize the influence of weathering conditions, during usage, on chemicals/particles leakage from the garbage lid and as well on its safety to the environment. The applied research carried out within Bio-Uptake-Hop-On will generate a complementary indicator that will benchmark the toxicity of developed materials and products to feed the Life Cycle Assessment to be carried out in WP1 and will assist WP5 on the selection of the bio-based materials and products that are more eco-friendly and safer for Humans, while keeping their functionality. By contributing to the development of materials and products with lower environmental and human impacts Bio-Uptake-Hop-On will support achieving Green Deal targets, namely those related with Zero pollution.