The production of new protein sources is necessary to sustain the worldwide consumption. Among them, oilseed proteins are good candidates due to their good amino acid (AA) composition and the important agricultural surfaces allocated to these crops in Europa and in France, especially rapeseed and sunflower. We will study the potential of theses crops, as well as flaxseed which outlets are increasing, to provide protein of good quality for human nutrition. For this purpose, several barriers have to be knocked down. On a technological point of view, protein extraction processes are not optimized and the incorporation of oilseed proteins in consumable food is few addressed. On a nutritional point of view, there are almost no data in humans related to the quality of these proteins. Our project will address these questions in three steps connected to each other. (1) Extraction processes will be optimized to increase the extraction yield, to remove compounds that can limit their interest for food products and to lower the volumes of effluents to achieve the most sustainable process as possible. (2) Assays of food enrichment will be implemented to obtain acceptable food products that can be produced at industrial scale. (3) Protein quality in these food products will be measured in vivo in humans using a novel dual tracer method for determining AA bioavailability. This method has been proposed by a FAO committee expert but was not implemented yet and will be developed in this project. The principle is to mix in an experimental meal a standard 13C protein (such as algae) together with the intrinsically labelled oilseed proteins. The blood 15N/13C or 2H/13C ratio will be compared to the ratio of the same AA in the meal and amodification of this ratio will reflect a modification of the AA bioavailability. The project will be conducted on a multistep mode, including upstream technological work to optimize protein extraction and purification, to formulate acceptable food products and to label the plants. As a result, experimental meals incorporating the intrinsically labelled proteins will be used for bioavailability in vivo studies. This project will bring the first validation of this non-invasive method for AA bioavailability, as well as the first data on protein and AA bioavailability of oilseeds, rapeseed, sunflower and flaxseed. The consortium is complementary and brings all the expertise necessary for the project: oilseed agronomy (Terres Inovia), oilseed ingredient production (Avril), plant protein biochemistry (LRGP) and in vivo protein quality assessment (PNCA). The project will provide basic and applied integrated additional knowledge related to agriculture, food sciences, food processing and human nutrition that will support local oilseed crop and oilseed protein development and use. This project will bring new data on the sustainability of oilseed proteins and their interest in human nutrition. It will favor the emergence of new sectors for exploitation of oilseed proteins in human nutrition. ProDige will assess the economic interest of greater crop diversification, and thus the opportunities of the diversification of species for agro-industrial strategies and consumer demands. This represents an important issue since rapeseed and sunflower represent a production of 1,2 million tons of protein in France. The project will bring keys points to determine the feasibility of exploiting this already existing protein resource and of developing food products enriched in oilseed proteins in France and Europa.