A comprehensive landscape of mRNA translation, storage and decay is required to understand the control of protein production. In eukaryotes, many regulators of these processes condense in cytoplasmic droplets called P-bodies. In a pioneer study we showed that P-bodies are primarily involved in mRNA storage, raising new issues. What is the spatiotemporal dynamic of mRNA storage and translation? What controls the differential storage of alternative mRNA isoforms? Is storage in P-bodies crucial to cell physiology and does it play a role in the recently found cases of intellectual disability (ID) with defective P-bodies? The objective of this project is to address these questions in the context of the cell cycle, using global multiscale approaches ranging from the molecular to the cellular level. Built on the main scientific interests of three partners, it capitalizes on their recent achievements and unique expertise on RNP granules, single molecule imaging and bioinformatics technologies.