Additional file 2: Figure S2. of Progenitor potential of nkx6.1-expressing cells throughout zebrafish life and during beta cell regeneration

appsOther research productkeyboard_double_arrow_right Image 01 Jan 2015Publisher:Figshare

Authors: AurĂŠlie Ghaye; Bergemann, David; TarifeĂąo-Saldivia, Estefania; Flasse, Lydie; Berg, Virginie Von; Peers, Bernard; Voz, Marianne; +1 Authors

doi: 10.6084/m9.figshare.c.3639137_d7 , 10.6084/m9.figshare.c.3639137_d7.v1

Additional file 2: Figure S2. of Progenitor potential of nkx6.1-expressing cells throughout zebrafish life and during beta cell regeneration

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Abstract

The bacterial artificial chromosome (BAC) reporter line Tg (ascl1b:eGFP-creER T2 ) mirrors the expression of the endogenous ascl1b gene. A Schematic representation of the â 61 to +89 kb ascl1b:eGFP-2A-creER T2 BAC transgene. By BAC recombineering using galK selection, the eGFP-2A-creER T2 cassette is introduced into exon 1, replacing the beginning of the ascl1b open reading frame (aa 1 to 163). B Epifluorescence microscopy images of the immunodetection of GFP in 17-hpf Tg(ascl1b:eGFP-creER T2 ) embryos. C, D Visible WISH showing expression of endogenous ascl1b in a wild-type (WT) embryo (C) and of GFP in Tg(ascl1b:eGFP-creER T2 ) embryos (D) at 15 hpf. Lateral views with the anterior part to the left. P pancreas. (PNG 1068 kb)

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