The interface of synthetic biology and (bio)materials is a highly promising, yet underexplored field of research. It can play a key role in addressing one of the biggest questions to mankind, which is how life started and how non-living materials became living. If synthetic polymers are successfully integrated into living cells, it will become feasible to induce effects within cells by bio-orthogonal triggers, thereby, opening up new avenues to gain control over cells, i.e. over their development and behavior. The overarching goal of this project is to integrate biocatalytic controlled radical polymerizations (bioCRP) into living cells as a fundamentally new approach to interface biological systems with synthetic polymers and polymeric nanostructures. This project aims to achieve a fundamental understanding of what happens when amphiphilic block copolymers are synthesized and self-assembled directly within cells, and how this process can be steered to form nanostructures or semi-synthetic cell membranes. To this end, methods for intracellular bioCRP and polymerization-induced self-assembly will be developed and the intracellular self-assembly of block copolymers and their integration into the cell membrane will be studied.

Do you use mobile or web apps or have Internet of Things devices on your person, in your home or workplace? Have you thought about who developed the software that drives these apps and devices, what was their understanding of cyber security, how did they make design decisions that impact the cyber security of the resulting software, and what factors influenced their behaviour and design choices? Or perhaps you are one of the masses exploiting app development platforms and easy-to-program hardware devices such as Arduino and Raspberry Pi to develop applications and deploy them for personal use or distribute them to millions of people around the world? How do you make cyber security decisions when you write software? Do you consciously think about the security implications of your design choices, or are there other factors that are more critical? What will help you achieve your goals from the software that you are developing while ensuring that it is not vulnerable to attacks by malicious actors? This project aims to develop a deep foundational understanding of these issues. We recognise that developing software is no longer the preserve for the select few with deep technical skills, training, and knowledge. A wide range of people from diverse backgrounds are increasingly developing software for mobile and web apps and for programmable consumer devices. This diversity of developers is at the heart of many innovations in the digital economy. The software they produce can be, and is, deployed across systems embedded in many aspects of human activity, and is used by a global user base. However, little is currently understood about the security behaviours and decision-making processes of 'the masses' engaged in software development. We refer to these masses by the pseudonym 'Johnny' - based on a seminal work by Whitten and Tygar where they highlighted the challenges faced by Johnny, the prototypical user of encryption. In this project we aim to tackle the challenges faced by Johnny in a contemporary setting beyond encryption. We focus on the Johnnys with diverse backgrounds, know-how and cyber security expertise who can, and are, developing software used, potentially, by millions worldwide. Drawing on a research team of experts in cyber security, software engineering, and psychology, our aim in this project is to conduct empirically-grounded research to better understand the security implications of Johnny's behaviours and practices and develop effective support for secure software development by Johnny. We propose to achieve this by uncovering and characterising the security vulnerabilities that Johnny tends to introduce, by analysing how and why these vulnerabilities are introduced, and by identifying and evaluating a range of interventions to improve Johnny's security behaviours during software development. We will do this in collaboration with eminent international research partners, drawn from leading research and practitioner organisations around the world. This project will be the first to study the inter-relationship between the cognitive and social processes that shape Johnny's cyber security decisions, their impact on the security of the resultant software and the novel interventions that may steer Johnny towards more effective cyber security decisions during software development.

Do you use mobile or web apps or have Internet of Things devices on your person, in your home or workplace? Have you thought about who developed the software that drives these apps and devices, what was their understanding of cyber security, how did they make design decisions that impact the cyber security of the resulting software, and what factors influenced their behaviour and design choices? Or perhaps you are one of the masses exploiting app development platforms and easy-to-program hardware devices such as Arduino and Raspberry Pi to develop applications and deploy them for personal use or distribute them to millions of people around the world? How do you make cyber security decisions when you write software? Do you consciously think about the security implications of your design choices, or are there other factors that are more critical? What will help you achieve your goals from the software that you are developing while ensuring that it is not vulnerable to attacks by malicious actors? This project aims to develop a deep foundational understanding of these issues. We recognise that developing software is no longer the preserve for the select few with deep technical skills, training, and knowledge. A wide range of people from diverse backgrounds are increasingly developing software for mobile and web apps and for programmable consumer devices. This diversity of developers is at the heart of many innovations in the digital economy. The software they produce can be, and is, deployed across systems embedded in many aspects of human activity, and is used by a global user base. However, little is currently understood about the security behaviours and decision-making processes of 'the masses' engaged in software development. We refer to these masses by the pseudonym 'Johnny' - based on a seminal work by Whitten and Tygar where they highlighted the challenges faced by Johnny, the prototypical user of encryption. In this project we aim to tackle the challenges faced by Johnny in a contemporary setting beyond encryption. We focus on the Johnnys with diverse backgrounds, know-how and cyber security expertise who can, and are, developing software used, potentially, by millions worldwide. Drawing on a research team of experts in cyber security, software engineering, and psychology, our aim in this project is to conduct empirically-grounded research to better understand the security implications of Johnny's behaviours and practices and develop effective support for secure software development by Johnny. We propose to achieve this by uncovering and characterising the security vulnerabilities that Johnny tends to introduce, by analysing how and why these vulnerabilities are introduced, and by identifying and evaluating a range of interventions to improve Johnny's security behaviours during software development. We will do this in collaboration with eminent international research partners, drawn from leading research and practitioner organisations around the world. This project will be the first to study the inter-relationship between the cognitive and social processes that shape Johnny's cyber security decisions, their impact on the security of the resultant software and the novel interventions that may steer Johnny towards more effective cyber security decisions during software development.

How liquids wet solid surfaces is of fundamental importance to a wide-range of scientific disciplines and technological applications from creating thin films on semiconductor wafers, through adhesion and coating of surfaces, to effective droplet deposition and mixing on DNA microarrays. Electrostatic fields can alter how effectively a liquid wets a solid surface. In recent years uniform electric fields have been used to control and manipulate droplets of conducting (ion containing) liquids, typically a salt solution, by using the liquid-solid contact area as one electrode in a capacitive structure - so called electrowetting. This has led to new voltage controlled variable focus liquid lenses, liquid-based electronic paper and droplet-based microfluidic systems. The key to electrowetting is the ability of an applied voltage to reversibly increase the effective hydrophilicity of a solid surface and reduce the contact angle of the droplet without altering the surface chemistry. However, many liquids of interest are not conducting and the need for a sandwich-style capacitive structure and direct physical contact to the liquid limits its range of applicability. In this project we create a new method of controlling hydrophilicity and oleophilicity of materials by using the dielectric properties of liquids, but with the effects localized to an interface. Unllike electrowetting which focuses on the ions, our method focuses on the dipoles in a liquid. Using a non-uniform electric field generates unequal forces on the two ends of the dipole. The resulting dielectrophoretic force can result in movement and redistribution of the liquid into the areas of highest field gradient. The basis of our project is the understanding that when the liquid has solid-liquid, liquid-vapor or liquid-liquid interfaces, dielectric energy changes can be coupled to surface free energy changes. With a suitable decaying electric field, the effects of liquid dielectrophoresis can be confined to either the solid-liquid interface or to the liquid-vapor (or liquid-liquid) interface and can be used with a non-conducting liquid. By using microfabricated interdigitated electrodes a decaying, and hence non uniform, electric field can be created above a solid surface. For a droplet thicker than the decay length of the electric field, the major change of the surface energy compensating liquid dielectrophoretic energy changes is via a change in the contact area with a solid and so this can be a method of reversibly controlling the contact angle and, hence, the hydro- and oleo- philicity of a surface. For a thin liquid film the major change of the surface energy compensating liquid dielectrophoretic energy changes is via a change in the shape of the liquid-vapor (or liquid-liquid) interface and so, in this case, it becomes a method for shaping a liquid surface. In this method of localizing the effects of liquid dielectrophoresis to an interface the contrast to electrowetting is that, 1. the electric fields are non-uniform; 2. the electric fields are generated by surface microfabricated co-planar rather than sandwich electrode structures; 3. the forces act upon the dipoles in the liquids, which can therefore be non-conducting (or conducting), rather than upon ions of conducting liquids; 4. the method does not suffer from saturation of the contact angle and so can be used to produce liquid films. The research in this project seeks to establish an approach to wetting that allows conducting and non-conducting liquids to be manipulated using electric fields in a manner complementary to electrowetting. The project will provide the understanding needed to allow future development of novel droplet microfluidic, liquid microactuation, liquid-based optics and displays. The project includes industrial partners who have expertise in the development and commercialisation of microfluidic liquid handling, lab-on-chip devices, display devices and optofluidic systems.

Molecular communication (MC) provides a way for nano/microdevices to communicate information over distance via chemical signals in nanometer to micrometer scale environments. The successful realization of MC will allow its future main applications, including drug delivery and environmental monitoring. The main hindrance for the MC application stands in the lack of nano/micro-devices capable of processing the time-varying chemical concentration signals in the biochemical environment. One promising solution is to design and implement programmable digital and analog building blocks, as they are fundamental building blocks for the signal processing at MC transceivers. With two existing approaches in realizing these building blocks, namely, biological circuits and chemical circuits, synthesizing biological circuits faces challenges such as slow speed, unreliability, and non-scalability, which motivates us to design novel chemical circuits-based functions for rapid prototyping and testing communication systems. Conventional chemical circuits designs are mainly based on chemical reaction networks (CRNs) to achieve various concentration transformation during the steady state from the input to the output with all chemical reactions occurring in same "point" location. This kind of design does not fit for the time-varying signals in communication system due to that the temporal information can be invisible to even state-of-the-art molecular sensors with high chemical specificity that respond only to the total amount of the signaling molecules. Thus, this project aims to design the chemical reaction-based microfluidic MC prototypes with time-varying chemical signal processing functionalities, including modulation and demodulation, encoding and decoding, emission and detection. This also facilitates the microfluidic drug delivery prototype design and cancer cell on chip testing under time-varying drug concentration signal. This project has the ambitious vision to develop novel time-varying chemical concentration signal processing methodology for microfluidic MC and microfluidic drug delivery. In the long run, 1) our microfluidic MC results will enable the implementation of MC functionality into nanoscale machines, by downsizing the proposed components through the utilization of nanomaterials with fluidic properties, and by translating the functional chemistry into biological circuit designs; 2) our microfluidic drug delivery results will revolutionize the conventional drug delivery testing approach by enabling ICT technologies for novel in-vitro microfluidics for drug delivery, allowing rapid measurement of therapeutic effect, toxicology, to reduce development costs and minimize the use of animal models.