SYMSITES?s main objective is to implement regional industrial urban symbiosis in four European regions different in social economic, and environment aspects, from the north Denmark, through the mid Austria to the south Spain and Greece. The four EcoSites will use the same technologies for wastewater and waste treatment and energy production and cycling, enabling a clean comparison of the EcoSite impacts. The four EcoSites will generate virtuous circles of energy, treated waste and wastewater streams between urban and industrial entities. The enabling technologies to be developed for waste and wastewater (WW) co-treatment, are: a newly developed IT Regional Management platform (ITRMP) including novel IIoTs and Social Decision support System (SDSS) to manage efficiently the I-U symbiosis; an anaerobic bioreactor(AnMBR) with advanced membrane coupled with a tertiary treatment, to be installed at the four EcoSites for a clean comparison of all impacts not directly influenced by different technologies. The aim is A) to achieve near-zero GHG emissions or CO2 negative footprint; B) use of bio-waste and non-recyclable wastes (NRW) to produce energy and reusable water as well as high-value new resources (HVNR), thus reducing the waste generation by ~50%; LCA and LCC studies and the quantity prevented from transportation (Ton-Kilometer units) and from landfilling (Ton/m3 units) will evaluate the costs and environmental impact. Dedicated tools will be developed to spread he I-US concept within Europe. These include: virtual demonstration of replication potential in other regions by setting up a network amongst waste associations ' facilitation services for implementing symbiotic processes; actions to facilitate relations and to involve the local community actors and establishment of a social innovation non profit spin-off involving tools and business models for streams exchange in a dynamic production.