Species populations are connected to each other through both movement of adults (migration) and eggs, larvae and juveniles (dispersal). If populations become isolated from one another (i.e. are no longer connected), then through genetic mutation, drift and natural selection, they may become so different that they evolve into new biological species. Understanding how populations become isolated is critical to understanding the process of speciation. In the marine environment many species do not move as adults (e.g. corals) or move very slowly (sea urchins). This means that for different adult populations to remain connected they rely on dispersal of early life history stages. Most marine species have a larval stage that lives in the plankton for a period of time, moving with the currents, before settling in a new area. It is larval dispersal that keeps distant populations connected. So understanding patterns of larval dispersal is important to understanding connectivity. In the deep-sea (>200m) the bathyal region of the continental slope has been identified as supporting high species richness and being an area where the rate of origination of new species may also be high. The reasons for this are not clear, but given the importance of connectivity to population isolation and speciation, it follows that the key to understanding patterns of species diversity in this region lies in understanding connectivity. New research has suggested that because the speed of the currents that carry larvae decreases as you go deeper, larvae might not be able to travel as far, leading to a greater tendency for populations at bathyal depths to become isolated over a given distance, and thus increasing the chances of speciation. This study aims to test this theory by investigating how patterns of connectivity vary with depth. This will be done in 3 ways: 1) using genetic analysis (similar to DNA fingerprinting) to compare how related distant populations are and if they become less closely related as you go deeper, 2) using a model of ocean currents to simulate the movement of larvae between sites, and 3) to look at the range and abundance of species present at distant locations to see if those at shallower depths are more similar to each-other than those at bathyal depths. This research has important implications for the sustainable management of the marine environment. Humans increasingly rely on the marine environment to supply us with food, building materials, fuel, and to soak up carbon slowing the progress of human induced climate change. However, our increasing use of this environment is starting to affect is 'normal' functioning, affecting the processes that allow it to provide us with food, fuel, etc. To try to help protect and sustain these 'ecosystem functions', Governments all over the world are setting up networks of Marine Protected Areas (MPAs) to ensure against serious ecosystem disturbance and cascade effects resulting from overexploitation that ultimately impair ecosystem function. There are many questions to be answered when trying to set up an MPA network, but one important question is where to put them to make sure that the populations that live within them are not isolated from each other but are connected. This research will help answer this question in the deep sea, and thus help managers, governments and society ensure the long term health of the ocean.

It is well established that the ocean is of enormous importance as it has an impact on climate, weather, global food security, public health and the economy; however, currently the increasing pressure on the ocean results in unseen levels of pollution and alterations of globally important chemical cycles. From the coast to the deep sea the ocean floor is largely covered by loosely aggregated sediments. These sediments form one of the largest bioreactors on Earth and play a crucial role in the state and health of the marine environment as they convert, store and release chemical compounds that affect and control life. For example, they promote the production of potent greenhouse gases and are a major sink for oxygen, but also recycle nutrients and retain pollutants. These biogeochemical reactions lead to steep gradients of chemical compounds in the upper centimetre to decimetre of the sediments, which can be used to understand the processes proceeding in the sediment, their effects on the global biogeochemical cycles and their impact on the marine environment. However, with traditional analysis methods these gradients can often not be properly resolved, both spatially and temporally, and they are often disturbed during the collection of the sediment; in addition, these measurements are costly and time-consuming. In the SANDMAN project I will develop a new instrument to measure gradients of important biogeochemical compounds, such as nutrients (nitrate, phosphate), metals (iron) and carbonate system parameters (total alkalinity) directly within the seafloor sediment, in particular the porewater, by combining cutting-edge Lab-on-Chip sensors with deep sea platform technology that can operate in extreme environments in the oceans over longer periods of time. The Lab-On-Chip sensors, which use miniaturized standard laboratory analyses on an automated microfluidic platform, are developed at the National Oceanography Centre and only recently became available for longer-term applications. These sensors are ideal for measuring the chemistry of porewater directly in the sediment as they are very energy efficient and can be deployed for up to a year and only use very little sample volume, hence the steep gradients in the sediment can easily be resolved. During the SANDMAN project I will lead the sensor adaptation and adjustment of the hardware for conditions in sediments, the design of a fluid sampling system to separate the porewater from the solid phase of the sediment and the combination of these components in a unique seafloor instrument. The functionality of this instrument will first be tested in a controlled laboratory environment, then in a costal test station and afterwards it will be used to answer scientifically important questions about the processes linked to nutrient and metal recycling and carbon degradation in currently underexplored areas such as permeable costal sediments and deep-sea trenches. This unique observing instrument can transform our capacity for the urgently needed benthic biogeochemical analysis from a human-dependent, single-point and costly sampling to a technology-based long-term, high-quality and reliable approach for remote biogeochemical measurements. The SANDMAN system will be widely applicable from the coast to the deep sea and from pole to pole for marine monitoring and industrial applications. Thus it will pave the way to novel synoptic seafloor observations, providing data to support and inform stakeholders, such as government/non-governmental organisations, industries, scientists and the general public, on environmental health and potential hazards.

Considerable effort and money has been devoted to determining the ecological consequences of a wide range of interventions, which has resulted in an extensive literature. However, research shows that practitioners only rarely use this literature when making decisions as to which intervention to implement. Furthermore, many accepted beliefs in conservation practice are actually incorrect. Scientific results are traditionally published in academic journals. However, it is often difficult for practitioners to extract the pertinent information from these. The major problems are that most practitioners do not have access to the Web of Science or equivalent scientific search engines, it is often difficult to target the search for conservation interventions without producing vast numbers of irrelevent titles and many practitioners do not have the training to extract the conservation message from academic papers. Evidence-based medicine has revolutionised medical practice in that the collection, review, and dissemination of the evidence now underpins most medical practice. We suggest that conservation would benefit from a similar revolution and propose that evidence-based conservation should become a standard approach. In this model we envisage practitioners having easy access to summaries of the literature, that they would monitor the effectiveness of some interventions for which the evidence is weak or ambiguous, that there would be reviews and meta analyses where there are numerous studies relating to one issue, and there would be synopses summarising the evidence for the major interventions. This proposal seeks to provide an open access database of the majority of the papers relating to the consequences for birds of conservation interventions. Syntheses of the consequences of a wide range of interventions will be a key output. Full use of the output will also require a change in approaches to conservation. The involvement of all the major organisations involved in bird conservation (BirdLife International - a partnership of over 100 national global bird conservation organisations, British Trust for Ornithology, Joint Nature Conservation Committee, Natural England, Royal Society for the Protection of Birds, Scottish Natural Heritage and World Conservation Monitoring Centre) will both ensure that the project is as required by practitioners but will also ensure that the results will be widely used both in the UK and internationally. Training in the use of evidence-based conservation will be provided through workshops in the UK, Africa and Asia and this work will also be promoted through stands at UK and international meetings. The longer term objective is to change global conservation practice so that the decisions effecting biodiversity are routinely based upon the scientific literature. The expectation is that we can build upon the work and experience of this project to expand it to incorporate all the major aspect of conservation in collaboration with a wide range of other organisation so that the use of evidence in decision making becomes standard practice This proposal would allow us to make a substantial step forward in achieving our objective of reforming global conservation practice.

A global demand for energy in parallel with concerns about global warming and energy security are motivating many nations to look for novel and sustainable sources of energy. At the same time the Oil ad Gas Industry is looking to decommission significant infrastructure as it comes to the end of its life cycle. There is a clear transition underway which brings challenges of infrastructure management. Among the issues raised by the offshore industries are those arising from the biological colonization of their structures. This project is aimed at describing the connectivity between structures and understanding the consequences for other sectors when structures are removed or added to the network in the norther North Sea. The project has been designed with several sectoral, governmental and industrial partners and there will be a strong emphasis on converting the scientific results into action at sea. The importance of colonization arises both from the need to make the developments efficient (to produce a reliable source of energy cost effectively) and to ensure the developments are environmentally acceptable. "Environmentally acceptable" covers a multitude of points, ranging from maintaining healthy sea life to avoiding conflicting with other sea users, including fishers who may have a prior claim on the development sites. The research in this project will be diverse to cover the many factors. A keystone of the project will be deployments of a Standard Monitoring System designed to facilitate data collection using practical and effective methods. That system centres on settling plates that will be progressively colonized by biofouling marine invertebrates. These organisms can impede the performance of the energy capturing devices, but can also be a foundation of thriving sea life. Structures including suitable niches can provide living space for larger organisms such as crabs and lobsters, adding to their "reef effect". The reef effect can be important to enhance marine life (biodiversity) but should also be beneficial to commercial fisheries, compensating fishers for some loss of access. However, there can also be dangers such as potentially adding to the spread of invasive species, and the research will also consider that. Ultimately, we want to find a way to ensure that offshore infrastructure is a positive addition to the marine environment and our research will be directed to that end.

Biodiversity on land provides huge benefits to humanity through carbon capture, resilient ecosystems, provision of food, and effects on human health. To manage and conserve our resources we need to understand how biodiversity is changing in response to the environment and human impacts. Moreover, we have implemented global targets to measure and protect nature through recent international agreements. Commonly used methods of reporting (e.g., datasets generated by citizen scientists) have limitations in terms of spatial, temporal and taxonomic resolution. This is problematic given that ecological communities are naturally dynamic systems in which both species and their interactions change, and these kinds of data are necessary to assess nature recovery. Both positive and negative human impacts on nature happen on relatively short timescales, but data are not collected with sufficient regularity for us to understand and manage these rapid changes. Coverage within datasets is biased towards popular animals (e.g., birds), with greater records coverage in densely inhabited areas. I have pioneered an approach that will deliver widespread, rapid understanding of biodiversity and ecological community dynamics on land. My initial work has shown that airborne environmental DNA (eDNA) is shed from animals, plants and fungi into the atmosphere, and that this broadly reflects the composition of the surrounding community. I have recently shown that this material is being continuously collected by an existing globally distributed infrastructure: air quality monitoring networks. These networks are filtering the air at daily or weekly intervals to measure particulates and pollutants but are also inadvertently collecting information on biodiversity. My overall aims are to understand how ecological communities are responding to human impacts globally and deliver technological solutions to the problem of scale in biodiversity monitoring using the air quality networks. I will do this by working with the environmental departments of four countries to analyse the molecular information from their air quality networks over three years. I will explain finescale temporal variation in species richness, turnover, community composition and ecological network "modules" (groups of tightly co-occurring species), and how these change over time according to environmental variation and human impacts. In addition, the recent COVID-19 pandemic offers the opportunity to quantify the effects of reducing human activity on biodiversity dynamics within the framework of a natural longitudinal experiment. While usual biodiversity monitoring was halted during these years, air quality monitoring continued, and these filters have been stored in long-term archives. I will work with an additional five countries (Switzerland and four countries in S America and Asia from the GAPS megacities project) to quantify the impacts of the "Anthropause" on biodiversity dynamics and ecological network properties. I will harness a multidisciplinary approach to further study the nature of airborne environmental DNA itself. Working with atmospheric scientists, I will study the particulate size range of airborne eDNA, in addition to the effects of sampling time and local weather conditions on samples. A combination of field experiments and particulate dispersal modelling will allow me to understand how far eDNA disperses from animal populations. These experiments will be used to inform other work packages in the FLF, but will also be of great interest to end-users implementing the technology. This work will have multiple beneficiaries from many sectors because society increasingly recognises that biodiversity preservation is both a cross-sector responsibility and challenge. I will work with policy stakeholders to explore how these data inform the UK's initiatives to protect and restore nature, monitor invasive species and forecast responses to future environmental changes.