Nanotechnologies are the new industrial revolution of this century. The dissemination of engineered nanomaterials (NMs) in the environment is suspected. Agricultural soils can be contaminated via nanopesticides and spreading of sewage sludge. It implies that crop plants (first gate into food chain) may be exposed to NMs at large scale. Within this project, we plan to study the fate of TiO2 NMs in an agricultural ecosystem and their impacts on digestive health. Indeed, TiO2 is a large volume manufactured chemical. With an integrative approach we will investigate the toxicity, transfer and transformation of these NMs from soil to crop plants, primary consumer (snails), and up to humans (epithelial intestinal cells and mice). The originality of our study is to link different multidisciplinary approaches (plant, animal and cell biologies, biophysics and modeling) to study different TiO2 NM transfer through the whole food chain. The main objective is to identify the risk for food safety related with NM dissemination and optimize a "safer by design" synthesis of TiO2 material.