Antimicrobials, commonly known as antibiotics, are becoming less effective because of resistance. Antibiotic resistance is when bacteria or other microbes change so that antibiotics no longer work to treat infections. Antibiotic resistance is a global problem that is being made worse by antibiotic overuse. We can combat antibiotic resistance by developing better antibiotics as well as improving the way we use existing ones. Patients will continue to need antibiotics, particularly to treat serious infections, like sepsis, so we need to improve how they are used. Right now, 'broad-spectrum' antibiotics, that kill a wide range of bacteria, are often given in high doses to ensure that enough antibiotic reaches the microbes at the site of infection. Much higher doses than would be needed if we could deliver antibiotics just at the site of infection are used. These antibiotics kill many of the beneficial 'resident' bacteria living in our bodies, which drives resistance. It would be much better if we could use a 'personalised medicine' approach where antibiotics are delivered locally, at the site of infection, at doses necessary to treat the problem. By giving lower doses of targeted treatment and avoiding exposure of the normal colonising bacteria to antibiotics, our vision is to improve health outcomes and reduce the selection of resistant microbes. Our project involves using tiny bubbles similar to those already used with ultrasound scanning to study the flow of blood through the heart and are currently being tested to treat cancers. These bubbles are given by injection into a vein. We propose to develop bubbles so that they can deliver antibiotics directly to a site of infection. The bubbles can also be burst using higher powered ultrasound, which is another possible way to kill bacteria. The bubbles are tiny, not much bigger than the bacteria, and will be coated with molecules that will allow the bubbles to stick to the surface of specific bacteria. This is known as 'molecular targeting'. By combining bubbles with ultrasound to trigger the release of antibiotics just at the site of infection, we aim to reduce the amount of antibiotics required to kill bacteria, without killing the helpful bacteria that live elsewhere in the body. Antibiotics often fail because the bacteria create their own local environment, the "biofilm", full of sticky chemicals, which also reduces the killing effects of antibiotics. Our approach will harness the energy released when an ultrasound pulse bursts bubbles to help drive drugs deep into this "biofilm" and hence help kill bacteria more effectively. In addition to getting more antibiotic into a biofilm, these drug-loaded bubbles will allow us to deliver new types of drugs, e.g. antimicrobial peptides (AMPs). AMPs are very effective at killing bacteria, but many cannot be given in the usual way, via a drip, into a vein to treat infections because they tend to be broken down in the blood before getting to the infection site. We can overcome this problem by loading the AMPs into tiny protective capsules attached to the bubbles and release them where/when they are required. Finally, we plan to investigate if bacteria can be released from their local biofilm environment using bubbles plus ultrasound. Here we will harness the mechanical energy released by bursting bubbles to break up the biofilm. The bacteria released from the biofilm are known as 'planktonic' and are more susceptible to conventional antibiotic treatments. In summary, we propose to: 1. Develop new targeting agents to bind bubbles to bacteria and new drug-loaded cargoes to kill bacteria/ destroy biofilms. 2. See if bubbles and ultrasound can be used together to deliver drugs into bacterial biofilms and kill bacteria more effectively. 3. Use our approaches to deliver drugs that cannot currently be used to treat patients because they are broken down in the blood.

Collisional and sliding contacts of two different materials are commonly associated with electric charge transfer, leading to charge accumulation. This causes an overwhelming number of handling and processing problems and explosion hazards, thereby degrading manufacturing efficiency and causing out of specification products and wastage. Examples are strong adhesion to containing walls and deposition in pipes, impairing flowability and aggravating segregation of components in a mixture, thereby upsetting formulations. It is common to experience highly active drugs filling up a spiral jet mill (thereby upsetting its functioning), components of a formulation preferentially depositing on grounded surfaces, getting concentration spikes of minor components of a formulation, poor powder spreading due to charging in additive manufacturing. In contrast, the phenomenon has been used to good effect in xerography and more recently for Tribo Electric Nano Generators (TENG). Despite being known for millennia, the triboelectrification phenomenon is not well understood and actually not predictable for non-metallic surfaces. The role of environmental humidity and temperature adds to the complexity. Considering its importance in advanced manufacturing of new materials, for which little material is initially available, a timely project with internationally leading-edge participation is proposed to tackle triboelectrification from a molecular level solid-state formation, right up to large scale manufacturing of active pharmaceutical ingredients and polymers. The project has seven industrial partners and six international collaborators from Japan, Brazil, Italy and Canada, contributing to seven work packages, each addressing a topic of scientific as well as industrial interest. The activities range from molecular solid-state level work function calculations by Density Functional Theory, to particle charge transfer characterisation by developing specialised instruments for charge distribution measurement and TENG, to unit operation level, including fast fluidisation and risers, pneumatic conveying and cyclone separation. The work is of strategic interest in manufacturing, ranging from pharmaceuticals, foods and plastics to additive manufacturing. It will have a huge impact on manufacturing sustainability, as the mitigation of triboelectrification issues will have a notable reduction in wastage and environmental footprint, and on the performance and material optimisation for the fast growing new technology of TENG. The proposed programme will tackle six challenges as addressed in the Case for Support.

Ammonia, a highly hydrogenated molecule, has been identified as an important means to support a transition to hydrogen economy, as it can be used to store and distribute hydrogen easily because of the already existing infrastructure for transport and storage of ammonia. If hydrogen is to be extracted from ammonia at the point of use, the thermo-catalysis of ammonia back to hydrogen requires a high amount of energy. Preferably ammonia is used directly as a carbon-free liquid fuel for combustion engines in power generation, marine vessels and long-haul vehicles where batteries cannot be used due to their low energy density (hence large volume and weight), high cost and long charging times. However, the significantly lower energy density (as measured by calorific value) of ammonia requires much larger fuel storage space and weight to be used. More importantly, the direct application of ammonia in combustion engines suffers from incomplete combustion and poor engine performance due to ammonia's higher ignition energy, higher auto-ignition temperature as well as significantly lower flame speed. In order to address the aforementioned challenges of ammonia and hydrogen for their applications in transport, a new type of liquid ammonia blended with hydrogen will be researched and demonstrated in this project with advanced modelling and experimental techniques. The proposed novel fuel has both ammonia and hydrogen molecules, and will enable (1) immediate and wider use of carbon free ammonia and hydrogen in existing engines, particularly for long haul vehicles, marine vessels and power generators, (2) significantly improved engine performance and lower emissions through increased energy density, faster and complete combustion. Therefore, the developed liquid ammonia blended with hydrogen would enable an immediate, cost-effective and 100% reduction in CO2 emissions to achieve net zero target in long haul transport, shipping, and power generation sectors by and beyond 2050 that will be difficult to achieve with existing technologies in use or in development.

More than 80% of world energy today is provided by thermal power systems through combustion of fossil fuels. Because of their higher energy density and the extensive infrastructure for their supply, liquid fuels will remain the dominant energy source for transport for at least next few decades according to 2019 BP Energy Outlook report. In order to decarbonise the transport sector, the Intergovernmental Panel on Climate Change highlights the important role that biofuels and other alternative fuels such as hydrogen and e-fuels could, in some scenarios provide over 50% of transport energy by 2050. The importance of the renewable transport fuel is also recognized by the UK Government's revised Renewable Transport Fuel Obligation published in April 2018 which sets out the targeted amount of biofuels to 12.4% to be added to regular pump fuel by 2032. In practice, there are several obstacles which hinder the application of low-carbon and zero-carbon fuels. As a zero-carbon fuel, hydrogen can be produced and used as an effective energy storage and energy carrier at solar and wind farms. But its storage and transport remain a significant challenge for its wider usage in engines due to the complexity and substantial cost of setting up multiple fuel supply infrastructure and on-board fuelling systems. Although the low-carbon renewable liquid fuels, such as ethanol and methanol produced from hydrogen and CO2, can be used with the existing fuel supply systems, the significantly lower energy density, which is about half of that of gasoline/diesel, makes them unfavourable to be directly applied in the existing engines for various applications (e.g. automotive, flying cars, light aircraft, heavy duty vehicles, etc.) with high requirements on power density. Whilst there is a drive to move towards electrification to meet the reduction of the carbon emissions, it is vital to innovate developments in advanced hybrid electrical and engine powertrain to provide additional options for future low-carbon transport. This research aims to carry out ground-breaking research on three innovative technologies covering both fuels and propulsion systems: nanobubble fuels and Nano-FUGEN system, fuel-flexible BUSDICE and DeFFEG system. The technologies either in isolation or as a hybrid have the potential to make a major contribution in addressing the challenge of decarbonising the transport sector. At first, I will explore how the nanobubble fuel (nano-fuel) concept can be used as a carrier for renewable gas fuels in liquid fuels in the form of nanobubbles. The technology can be implemented with minimal new development to the combustions engines and hence has the potential to make immediate impact on reducing CO2 emissions through better engine efficiency and increased usage of renewable energy. Secondly, a novel 2-stroke fuel-flexible BUSDICE (Boosted Uniflow Scavenged Direct Injection Combustion Engine) concept will be systematically researched and will involve development work for adapting to be used with both conventional fossil fuels and low-carbon renewable fuels (e.g. ethanol and methanol) and simultaneously achieve superior power performance and ultra-low emissions. At last, based on the developed BUSDICE concept, a Dedicated Fuel-Flexible Engine Generator (DeFFEG) will be further developed by integrating a linear generator and a gas spring chamber, therefore enabling advanced electrification and hybridisation for a range of applications, including automotive, aviation and marine industries. Overall, the proposed project is an ambitious and innovative study on the fundamentals and applications of the proposed fuel and propulsion technologies. The research not only has great potential to bring about new and fruitful academic research areas, but also will help to develop next-generation fuel and propulsion technologies towards meeting Government ambitions targets for the future low-carbon and zero-carbon transport.

Increasing emission levels of air pollution and greenhouse gases (GHGs) in large urban areas have become a great global concern due to their detrimental impact on human health, climate and the entire ecosystem. In order to cut emission levels, mitigation strategies are in place, however, to evaluate the effectiveness of these mitigation measures, the first step will be to improve the air quality (AQ) monitoring networks by deploying high density and high precision sensor networks to accurately capture spatial variability and emission hotspots in real-time. The traditional and more accurate air quality monitoring instrumentation are large, complex and costly, and hence are only sparsely deployed which provide accurate data but only in few locations, not providing enough information to protect the health of the population or to accurately evaluate the mitigation strategies. The emergence of low-cost sensors (LCS) within the last decade enabled observations at high spatial resolution in real-time, however, due to their poor selectivity, their measurement data is highly dependent on atmospheric composition, and also on meteorological conditions that the data generated by these platforms are of poor quality. In this fellowship, I will develop the first low-cost and high precision air pollution monitor based on photonic integrated circuits (PICs) for the next generation air quality monitoring networks. Photonic integration allows hundreds of photonic components to be fabricated on a single chip, and this step-change in technology will deliver a low-cost, on-chip, versatile instrumentation, stabilised to metrological precision that can be deployed in high density networks to accurately monitor a wide range of pollutants within industrial cities with high spatial and temporal resolution. The captured data can be transferred to the cloud servers over the existing mobile networks from which the users can easily monitor air quality with high accuracy at any time and from anywhere. The proposed instrumentation can also be deployed in balloon and satellite missions for in-situ probing of the constituents of the upper atmosphere, aiding the study of complex atmospheric processes to understand its influence on climate change. EPSRC Open Fellowship will enable me to consolidate my expertise gained over the years in industry and academia and gain my research independence. During these five years, I will have established myself to lead a team of 3 -5 researchers and will have enhanced my research output in novel photonic integrated solutions to combat the challenges faced today. This will aid me to be more competitive in applying for traditional Grants to extend my research portfolio and my research team, and become a leader in this field of research. In 10 years, my vision will be to exploit photonic integration technology for wider applications, including medical imaging, material science and non-destructive testing, and provide outstanding training opportunities to research students and early career researchers who will grow to be future academic and industrial leaders in science and engineering in the UK.