Plants are intimately associated with a diversity of beneficial microorganisms in their root zone, some of which can enhance the plant’s resistance to insect pests. Thus, the use of Microbe-induced Resistance (MiR) to reduce pest losses in agriculture has emerged as a highly promising possibility to improve crop resilience and reduce use of harmful pesticides. European companies have therefore started to develop and market beneficial microbes. However, MiR appears to be strongly context-dependent, with reduced benefits under certain biotic and abiotic conditions and in some crop varieties. Further, it is a challenge to deliver and ensure stable associations of beneficial microbes and plants, and avoid undesired effects on beneficial insects. Thus we absolutely must improve our understanding of MiR mechanisms and context-dependency, in order to improve context stability of MiR and promote the use of MiR for crop protection. Our MiRA project will train 15 ESRs in basic and applied research on context-dependency of MiR, mechanisms, and impacts on plant performance and other biocontrol organisms. We will use this understanding to improve our ability to predict the effectiveness of MiR under different conditions, to select plant and microbial strains with improved context-stability, and to develop better methods for the formulation of microbial inoculants and their application in agriculture. Finally, we will analyse economic prospects and constraints for MiR development and use. To achieve these goals, we have assembled a consortium of 11 academic institutions and 6 companies, including microbial inoculant producers and agricultural advisors, with complementary skills in basic and applied research and innovation. Our ESRs will be trained within this multi-sectoral interdisciplinary network for a future career in research, product and service development in European horticulture and agriculture, pushing boundaries in European research and innovation.

The Directive 2009/128/EC sets rules in EU for the sustainable use of pesticides to reduce the risks and impacts of pesticide use on people's health and the environment. Among the listed actions there is the promotion of low pesticide-input management including non-chemical methods. In parallel several chemical active ingredients have been banned because of toxicity concerns. The result is that growers are left with few control tools against pests. On the other hand most of the available alternative control methods have several limitations, especially in term of efficacy. Several new ideas are not reaching the industry and are confined in the academic world. The concept behind this EIT is to explore new approaches to identify new cutting edge solutions for pest control based on new non classical approaches in strict collaboration with industrial partner and to train 11 highly skilled early stage researchers (ESR) through a doctoral programme that integrates 5 academic research with concept-driven product development in 6 EU companies with a strong curriculum in development and innovation within a large interdisciplinary environment. Microorganisms are often used so far as replacement of chemical active ingredients. The innovative aspect of this EID is to base the new pest control solutions on interactions of microorganisms with plants and insects rather than using them as plant protection products. Microorganisms’ unsurpassed inclination towards the association with eukaryotic macro-organisms determines traits and qualities in the host that harbours them. Microbial symbionts’ ability to profoundly transform their living habitat paves the way for unexplored outlooks in the ability to use microbial symbioses as sustainable and renewable tools to improve production and quality in agriculture. Microorganisms are key players in shaping several insect’s semiochemicals, in particular kairomones indicating a food source or oviposition site for some insect species.

In ARISTO (the European Industry - Academia Network for RevIsing and Advancing the Assessment of the Soil Microbial TOxicity of Pesticides), leading universities and industrial partners join forces to perform a cutting edge research and doctorate training programme tackling the global challenge of minimising the environmental off-target effects of pesticides. The multi-sectoral approach of ARISTO, interlinking disciplines from soil microbiology, microbial ecology, environmental chemistry and risk assessment, will generate the new generation of Microbial Ecotoxicologists specialized in pesticides-soil microbes interactions. The research challenge of ARISTO is to produce benchmarking knowledge supporting the development of advanced tools and procedures, based on the response of key microbial indicator groups like ammonia-oxidizing microorganisms (AOM) and arbuscular mycorrhizal fungi (AMF), for the comprehensive assessment of the toxicity of pesticides on soil microorganisms. ARISTO offers doctorate fellows a challenging training programme build along 5 research objectives: (1) to develop pioneering in vitro tests, as a first conservative step, to assess the toxicity of pesticides on distinct AOM (ESR1) and AMF (ESR2) strains (2) to develop advanced experimental lab and field tests to assess the toxicity of pesticides on natural soil assemblages of AOM (ESR3) and AMF (ESR4), as a more realistic toxicity assessment step (3) to develop an ecosystem-level toxicity assessment: identify the response of soil microbial networks to pesticides (ESR5) and explore the impact of pesticides on microorganisms from different trophic-levels within the soil food-web (predator - prey) (ESR6) (4) to develop novel tools and procedures to determine the soil microbial toxicity of pesticide mixtures (ESR7) and biopesticides (ESR8) (5) to develop & validate advanced in silico tools for prioritizing transformation products (TPs) of pesticides with potential toxicity to soil microbes (ESR9)

We need to increase the crop yield while reducing pesticide and use of inorganic fertiliser to meet the challenges of world population growth and climate change. Plant endophytic microorganisms can improve plant yield and enhance plant tolerance to abiotic stress as well as to pathogens under experimental conditions, but these effects are often not sufficiently stable for practical application. How do we boost the stability and reliability of the positive effects of endophytes on plants? We need to understand the genetic basis of beneficial interactions between crops and endophytes and extent this basis exhibits phenotypic plasticity at all interaction levels from the cellular to the field environment. This requires increasing our knowledge of the molecular mechanisms underlying the effects of endophytes, including intra and inter-kingdom exchange and distribution of resources (nutrients), signalling and possibly regulation between and inside the partners, the mutual induced production of secondary metabolites and the environmental cues which influence crop-endophyte interactions. The genetic variation and its plasticity in host and microbe will be exploited in to establish crop breeding and inoculum production processes for boosting the establishment and stability of plant-microbe mutualisms to benefit crop development, stress tolerance, pathogen resistance and quality. In this project we will provide fundamental biological as well as practical knowledge about interactions between endophytes and plants. This improved understanding will pave the way for increased use of endophytes to improve sustainability and plant productivity in a reliable way. The participants in this project comprise many of the key institutions and industries working with these problems and provide a uniquely strong consortium to address the key issues. Furthermore, the consortium will train a new generation of scientists who have the insight and skills to continue this task in their careers.