Project Ô intends to demonstrate approaches and technologies to drive an integrated and symbiotic use of water within a specific area, putting together the needs of different users and waste water producers, involving regulators, service providers, civil society, industry and agriculture. The project seeks to apply the pillars of integrated water management (IWM) as a model for “water planning” (akin to spatial planning) and to demonstrate low cost, modular technologies that can be easily retrofitted into any water management infrastructure at district/plant level, hence enabling even small communities and SMEs to implement virtuous practices. Technologies and planning instruments complement each other as the first make possible the second and the latter can provide as example or even prescribe the former (and similar technologies allowing virtuous water use practices). Indeed the technologies support the regulators in implementing policy instruments, as foreseen by IWM, for convincing stakeholders (like developers and industry) to implement water efficiency strategies and could include instruments for e.g. rewarding virtuous behaviours (for example: advantageous water tariffs), planning regulations that award planning consent more swiftly or even prescribe the use of water from alternative sources (including recycling). Project Ô has in summary the overall objective of providing stakeholders (everybody using or regulating the use of water in an area) with a toolkit that enables them to plan the use of and utilise the resource water whatever its history and provenance, obtaining significant energy savings in terms of avoided treatment of water and waste water and release of pressure (quantity abstracted and pollution released) over green water sources. This overall objective will be demonstrated in up to four sites each in different Countries of Europe and in Israel, involving industries, aquaculture and agriculture as well as local authorities of different sizes.

Organisms across all kingdoms share several systems that are essential to life, one of the most central being protein synthesis. Living in a continuously changing environment, cells need to constantly respond to various environmental cues and change their protein landscape. In extreme cases, cells globally shut down protein synthesis and upregulate stress-protective proteins. Mechanisms of translational repression or selective enhancement of stress-induced proteins have been characterized, but their effects were demonstrated on an individual mRNA basis. Which target mRNAs are translationally regulated in response to different environmental cues, and what are the cis-regulatory elements involved, largely remain as open questions. Using ribosome footprint profiling, I recently discovered a novel mode of translational control in stress, underscoring the potential of new technologies to uncover novel regulatory mechanisms. But while transcription cis-regulatory elements have been thoroughly mapped in the past decade, and splicing regulatory elements are accumulating, the identification of translation cis-regulatory elements is lagging behind. Here I propose to crack the mammalian translation regulatory code, and close this long-standing gap. I present a novel interdisciplinary framework to comprehensively identify translation cis-regulatory elements, and map their mRNAs targets in a variety of cellular perturbations. Importantly, we plan to explore mechanisms underlying novel cis-regulatory elements, and create the first genome-wide functionally annotated translation regulatory code. The translation regulatory code will map targets of existing mechanisms and shed light on newly identified pathways that play a role in stress-induced translational control. The proposed project is an imperative stepping stone to understanding translational regulation by cis-regulatory elements, opening new avenues in the functional genomics research of translational control.