The goal of B4EST is to increase forest survival, health, resilience and productivity under climate change and natural disturbances, while maintaining genetic diversity and key ecological functions, and fostering a competitive EU bio-based economy. B4EST will provide forest tree breeders, forest managers and owners, and policy makers with: 1) better scientific knowledge on adaptation profiles and sustainable productivity, and added value of raw materials in important European tree species for forestry, 2) new and flexible adaptive tree breeding strategies, 3) tree genotypes of highly adaptive and economical value, 4) decision-support tools for the choice and use of Forest Reproductive Material (FRM) while balancing production, resilience and genetic diversity, including case studies developed with industrial partners, 5) integrative performance models to guide FRM deployment at stand and landscape level, 6) economic analyses of risks/benefits/costs, and 6) policy recommendations. B4EST will capitalise on the resources developed by past and current EU projects to produce -together with tree breeders, forest managers and owners, and the industry- operational solutions to better adapt forests to climate change and reinforce the competitiveness of the EU forest-based sector. To cover the geographical, economic and societal needs of forestry in the EU, B4EST will work with 8 (six native, two non-native) conifers and broadleaves with advanced breeding programmes (Norway spruce, Scots pine, maritime pine, poplars, Douglas-fir, eucalypts) or that are case studies of pest-threatened forests (ash) or valuable non-wood products (stone pine). Our approach will result in a high degree of data and knowledge integration, involving multiple and new target traits and their trade-offs; genomic information; temporal and spatial assessments in a wide range of environments; stakeholder demands; and forest owner and manager risk perception and acceptability of new breeding strategies.

Gene flow has long been considered to take place within species only but we now realize that it often occurs between species as well. We still don’t know, however, how much gene flow effectively affects the genome of hybridizing species in the late stage of speciation. Such hybridization may be a source of adaptive genetic variation via the transfer of adaptations from the genome of one species to another, a phenomenon called “adaptive introgression”. While there are a few known prominent examples, its overall importance for adaptation is still largely unknown. In this project, we address the following main questions: i) how much of the genome is affected by introgression and ii) what proportion of introgression is adaptive? We have selected the Iberian wall lizard species complex because they have accumulated substantial genomic divergence; in spite of strong barriers to gene flow, nuclear and mitochondrial introgression still occurs; a transcriptome from our model and a reference genome from a close relative are available and we know their distribution, ecology and climatic niches. Last, we already have over 1000 tissue samples so sampling will be limited to additional locations specifically targeted for this project. To achieve this, we will use whole-genome sequencing to quantify the proportion of the genome affected by admixture. We will then quantify which proportion of introgressed genome is better explained by positive selection. To do so, instead of trying to pinpoint which genes have been experienced adaptive introgression, we will develop a theoretical study using simulations to establish the neutral variance in admixture rates among loci then estimate which proportion of admixture events cannot be explained by neutral processes (see Task 4). To overcome some of the limits of purely genomic approaches, we also propose an ecological test of the adaptation hypothesis based on candidate genes for climatic adaptation (mitochondrial DNA and the nuclear genes of the OXPHOS chain) in populations living in contrasted climatic conditions (Task 5). We will sample several pairs of populations within each species, each pair being composed of one population located in highly suitable climatic areas and the other in areas where climatic conditions resemble the climatic niche of a hybridizing (donor) species. Finding more loci that have been subjected to introgression in areas that resemble more the climatic conditions of the “donor” species would support the role of adaptive introgression. Tasks 1 & 2 We will model the current realized climatic niche in all lineages. We will then sample populations in locations (2 per species) of high climatic suitability for the focal species and in the heart of their distribution and in locations (2 per species) where climatic suitability is higher for the other species that hybridizes with the focal species. Task 3 We will obtain WGS data from 3 individuals in each sampled population (6 per species, 6 species). Task 4 We will establish by simulation the neutral variance in introgression levels between nuclear loci in the absence of selection. This should give us the limits of the variation that can be reached between loci in terms of introgression level in absence of selection and allow developing methodological tools to identify loci that have been subject to adaptive introgression. Task 5 We will identify introgressed genomic regions using already published methods then apply results from task 4 to test our idea that the proportion of loci affected by adaptive introgression (the proportion of high-frequency introgressed alleles that cannot be explained by neutral processes) is higher in areas where climatic conditions are closer to the climatic niche of the species which “gave” its genes through introgression, both for the whole genome data and for the OXPHOS genes and mtDNA.

The overall objective of GALILEO is to rely on genuine Multi-Actor Approaches (MAA) to co-develop context-specific, people-centered agroforestry innovations in representative agro-pastoral, agroforestry, and agro-silvo-pastoral systems from Sub-Saharan Africa (SSA). The aim is to promote agroforestry as leverage to significantly improve agricultural, household, and climate change adaptation and mitigation performances and to enhance biodiversity in SSA. We build upon 8 agroforestry Living Labs (LLs: local scale and actors), 4 national and 1 regional Innovation Platforms (IPs), set up across 4 AU SSA countries. Our LLs are set in semi-arid zones of Senegal and Kenya and normally humid but drought-prone zones of Ghana and Cameroon thus comparing and covering a large range of SSA conditions. Through MMA, we co-construct potentially adoptable scenarios ex-ante with Innovator, Target, and Control actors in our LLs, then implement, assess, and compare performances in their pilot plots during the whole project. We use field observations also to calibrate process models, able to simulate under future CC scenarios. After full multi-criteria and trade-off analysis, we finally co-select the most effective scenarios ex-post. We thus rely on transdisciplinary research, providing qualitative and quantitative data on the biophysical, socio-economic, and environmental performances. Such adoptable agroforestry innovations will also enable farmers/pastoralists and stakeholders to diversify their incomes from new agroforestry value chains, of which 2 are GALILEO-original. They will also benefit from carbon farming and payment for ecosystem services opportunities. Through our IPs, we also engage in solid MAA collaborations and policy dialogues to first identify bottlenecks and second elaborate guidelines, and policy recommendations, helping towards strengthening their local innovation ecosystems, under a favorable institutional and policy framework.

Tools and institutions of international cooperation built up after the 2nd World War seemed to be underperforming when facing global threats on the environment, the importance of which is underlined by many recent scientific reports. International Law must go beyond its traditional purpose of supporting inter-state cooperation since it must now define rules and standards likely to be incorporated into the national legislation to help coordinate, if not harmonise, national environmental legal and policy frameworks. Beyond this remarkable expansion of international Law (some say treaty congestion) these institutions and instruments have been significantly transformed to cope with the above-mentioned threats with some new kinds of expert advice, the development of multilateral treaty making, some new types of norms, the growing role of private actors, and the development of new forms of international control --both public and private. However the global environmental governance remains fragmented. Without a world executive and legislative power, there is a proliferation on the international scene of norm producers and disseminators. The creation of a World Environmental Organisation is still in limbo and it is also disputable whether such an organisation would suffice to integrate the “multiple sites of governance” [Snyder, 2010]. The latter are loosely articulated, among themselves and with the other regulation mechanisms in domains such as trade, investment or human rights and so on, although some research points at the burgeoning architecture mixing or alternating synergy, cooperation and conflict relations between different regimes [Biermann, 2009]. The international governance of the environment was first understood through international regime analysis, where regimes are defined as sets of principles, norms, rules, and procedures, which shape the behaviour of actors in a specific area. In practice this corresponds to international conventions and subordinate treaties. More recently though, it was suggested that these regimes are embedded in some more elaborated settings labelled “regime complexes”. These are made of three or more international regimes addressing some different issues within a common domain, which not only co-exist by also interact on substance or at operational level, without being formally coordinated, and by working alongside with other governance mechanisms involving private corporations and NGOs. On the basis of this conceptualisation that saddles International Law, International Relations, Political Science, Political Economy and Sociology, this research project aims to analyse the enabling conditions, the forms and the impacts of norm circulation within actor networks by focusing on two important regime complexes, biodiversity and climate change. The fragmentation diagnosis being well established, it seems important to analyse these process through actor network analysis and focus on circulation of norms and actors. The core concept here is the “permeability” of the various elements of the regime complex, how circulation takes place and what are the impacts on the complex itself, and beyond on international governance as a whole.