Agro-industrial residual biomass, side streams and food production by-products like legumes, fungi and coffee are potential sources of valuable ingredients even though the routes for their exploitation are still at an early stage. Pursuing the ambition of achieving prolific valorization of untapped biomass streams, the project R%D&I activities and partners of Prolific have been positioned around a central innovation cycle that is mainly driven by industrial end-users who exactly know the needs of their customers and the technical constraints and industrial environment in their respective sector. The PROLIFIC project will apply a range of processing technologies to recover significant amounts of proteins/peptides and other value added compounds (e.g. carotenoids, phenols, caffeine and fibers) from industrial processing residues of legumes (seeds of peas, beans and chickpea), fungi (cuttings and mycelia of different species) and coffee (silver skin residue and not compliant roasted seeds). The economically and environmentally sustainable extraction, enzymatic modification, and conditioning techniques will be upscaled in industrially relevant environment. This will enable the production of consequent amounts of compounds and fractions necessary for the production of 16 product prototypes for the food, feed, packaging and cosmetic sectors. The Prolific project will assess the environmental, societal, ethical, safety, and regulatory issues at each step of the targeted value chains. SME and large companies strive at achieving competitive biomass exploitation and attracting when needed additional investors. The industrial partners business strategies for the future market penetration starts with the clear identification of the customer needs and goes up to the preparation of give-aways of food and cosmetics prototyped products as a reward for filling in questionnaires about the Prolific products along with intensive dissemination and communication.