Located on AAFC's Central Experimental Farm, the Canadian National Collection of Insects, Arachnids and Nematodes (CNC) of Agriculture and Agri-Food Canada is one of the five largest collections of its kind in the world. It contains over 17 million specimens. The CNC is an active centre of scientific research and an invaluable national resource. It serves Canadian scientists who investigate problems in the fields of agriculture, biodiversity, genetics, biological control and other various forms of integrated pest management, and it is part of an international network for other researchers, educators and policy-makers. An increasingly important function of the Collection is to support science that will protect Canada's biodiversity, including reducing the billions of dollars lost annually to invasive species in agriculture, forestry, northern wilderness areas and other native habitats. CNC is working to digitize its holdings and make them accessible online. This dataset will be updated as this work progresses. Refer to our website for more information: https://agriculture.canada.ca/en/agricultural-science-and-innovation/agriculture-and-agri-food-research-centres-and-collections/canadian-national-collection-insects-arachnids-and-nematodes-cnc .