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NEON Biorepository Fish Collection (DNA Extracts)

Authors: NEON Biorepository Portal;

NEON Biorepository Fish Collection (DNA Extracts)

Abstract

This collection contains genetic extracts from select fish collected during fish sampling at aquatic sites (NEON sample class: mam_barcoding_in.dnaSubsampleID.fsh). Fish are sampled using a combination of electrofishing, gill-nets and mini-fyke nets in wadeable streams and lakes. Fin clips are collected from 5-10 individuals of a target species. Tissues are preserved in an appropriate tissue vial and shipped to an external lab. DNA is extracted and target sequences amplified via PCR. Barcodes of cytochrome oxidase are generated per specimen. Genetic extracts are stored dehydrated in 96-well plates from the Canadian Centre for DNA Barcoding are archived in the NEON Biorepository at -80 degrees Celsius. When eluted into 20 uL, concentrations of 5-30 ng/uL are expected. See related links below for protocols and NEON related data products. Information about the Canadian Centre for DNA Barcoding can be found at https://ccdb.ca/.

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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