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Transcriptional signature of SPCH mutants compromised in the bHLH domain in response to brassinosteroids

Transcriptional signature of SPCH mutants compromised in the bHLH domain in response to brassinosteroids

Abstract

In order to describe the molecular phenotype of spch-5 and to explore possible changes in gene expression underlying the effects on its epidermal development of BL treatment, we performed a microarray-based transcriptomic analysis. Using 3-days old seedlings grown in the absence or presence of BL, we compared the transcriptomic profiles of wild type (Col-0) and spch-5 plants. In addition, we analyzed a SPCHPPP-complemented spch-3 line to identify gene expression changes distinct of spch-5 or common to the two SPCH variants. We thus obtained six data sets (3 genotypes in two growth conditions). Overall design: 3 days-old Arabidopsis plants grown in mock medium (DMSO) and brassinosteroids treatment (BL 50 nM) were used. Three genotypes were used: Col-0, spch-5 and SPCHPPP

Keywords

Transcriptomics

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